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During plant tissue culture growth sucrose acts as a fuel source for sustaining photomixotrophic metabolism (organisms can use different sources of energy and carbon), ensuring optimal development, although other important roles such as carbon precursor or signaling metabolite have more recently been highlighted. Sucrose is a very important part of nutrient medium as an energy source, since most plant cultures are unable to photosynthesize effectively owing to poorly developed cellular and tissue development, lack of chlorophyll, inadequate gas exchange and carbon dioxide in tissue culture vessels, etc.
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Plant research often involves growing new plants in a controlled environment. These may be plants that we have genetically altered in some way or may be plants of which we need many copies all exactly alike. These things can be accomplished through tissue culture of small tissue pieces from the plant of interest. These small pieces may come from a single mother plant or they may be the result of genetic transformation of single plant cells which are then encouraged to grow and to ultimately develop into a whole plant. Tissue culture techniques are often used for commercial production of plants as well as for plant research.
During autoclaving the medium sucrose is hydrolyzed to glucose and fructose, which are then used by the plant material for their growth. Fructose, if autoclaved is toxic. It has been found that a plant tissue culture medium containing glucose or fructose sterilized by autoclaving inhibits the growth of carrot root tissue cultures. More growth inhibition occurs when sugar and culture medium is autoclaved together. Other mono- or disaccharide and sugar alcohols like glucose, sorbitol, raffinose, etc., may be used depending upon plant species. Sucrose is still the best source of carbon followed by glucose, maltose, and raffinose; fructose was less effective and mannose and lactose were the least suitable. Carbohydrate sucrose is generally required to be present in addition to IAA before tracheid elements are differentiated in tissue cultures.
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